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  • Home Product Foreign distribution products 德國ibidi μ-Slide I Luer Glass Bottom
    μ-Slide I Luer Glass Bottom

    Model:80167 80177 80187 80197

    Brand:ibidi

    Specifications

    Introduction:Channel slides with a #1.5H glass bottom for flow applications; different heights and volumes available

    Adherent cells under flow conditions

    Cell culture (static or stop-flow)

    Simulation of blood vessels with endothelial cells

    High-resolution microscopy of living and fixed cells

    TIRF and super-resolution microscopy

    Want to know if you should use a glass or a polymer bottom for your application? Find out here.

    Because of the adhesive layer, the channel height of the μ-Slide I Luer Glass Bottom increases by 50 μm compared to the μ-Slide I Luer.

    Specifications

    Outer dimensions

    25.5 x 75.5 mm2 (w x l)

    Channel length

    50 mm

    Channel width

    5 mm

    Adapters

    Female Luer

    Volume per reservoir

    60 μl

    Growth area

    2.5 cm2

    Bottom: Glass coverslip No. 1.5H, selected quality, 170 μm +/- 5 μm

    μ-Slide I Luer Glass Bottom

    0.2

    0.4

    0.6

    0.8

    Channel height

    250 μm

    450 μm

    650 μm

    850 μm

    Channel volume

    62.5 μl

    112.5 μl

    162.5 μl

    212.5 μl

    Coating area

    5.2 cm2

    5.4 cm2

    5.6 cm2

    5.8 cm2

    *Standard format with thin coverslip bottom made from D 263 M Schott glass #1.5H (170 μm +/- 5 μm) for low or high magnification microscopy

    *Large observation area for microscopy

    *Defined shear stress and shear rate levels

    *Easy connection to tubes and pumps using Luer adapters

    *Fully compatible with the ibidi Pump System

    *May require coating to promote cell attachment

    *Also available as a μ-Slide I Luer with an ibidi Polymer Coverslip Bottom for superior cell growth

    The Principle of the μ-Slide I Luer Glass Bottom

    The Glass Bottom

    The μ-Slide I Luer Glass Bottom comes with a thin #1.5H Glass Coverslip Bottom that has the highest optical quality and is ideally suitable for high-resolution microscopy, TIRF, and super-resolution microscopy. To promote cell attachment, a surface coating might be required prior to cell seeding.

    Find more information and technical details about the coverslip bottom of the ibidi chambers here.

    Choosing the Right Channel Height for Flow Applications

    Which Experiment Are You Planning?

    Low channels are more suitable for flow applications.

    High channels are more suitable for static cell culture.

    For flow assays with small amounts of medium and high values of shear stress

    0.2 channel

    For a wide range of shear stress

    0.4 channel

    For controlling low values of shear stress < 2 dyne/cm2

    0.6 and 0.8 channels

    Select the ideal Perfusion Set for your flow application here.

    Cross Section of the Different Channel Heights

    * not recommended for static culture over more than 6 hours

    Experimental Example

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