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  • sticky-Slide Chemotaxis

    Model:80328 10812

    Brand:ibidi

    Specifications :10 (individually packed)

    Introduction:A chemotaxis bottomless slide used in 2D and 3D applications with a self-adhesive underside designed to allow self-assemblage of the slide setup Versatile – i.e., permits the mounting of a variety of bottom materials

    Applications:

    Inserting materials or tissue into chemotaxis chambers

    Keeping cell spheroids or tissue in a stable concentration gradient

    Chemotaxis of fast or slow migrating cells on either a 2D surface or in 3D gels

    Usable with a range of bottom materials, such as plastic sheets, silicon chips, and glass slides

    Specifications:

    Outer dimensions (w x l)25.5 x 75.5 mm2
    Chemotaxis chambers on slide3
    Volume per chamber130 μl
    Observation area2 x 1 mm2
    Height of reservoirs1.05 mm
    Total height with plugs12 mm
    Volume chemoattractant30 μl
    Bottomnone


    Technical Features:

    Bottomless slide

    Self-adhesive underside

    Biocompatible adhesive that has been cell culture tested

    Adheres to all flat surfaces, even wet surfaces

    Suitable coverslips available

    Specifications identical to those of the μ-Slide Chemotaxis, except that it has no bottom

    Placing Tissue Sample in the sticky-Slide Chemotaxis

    Application Example: Directed Invasion of MCF-7 Cancer Cells in an FCS Gradient

    This movie shows the invasion of MCF-7 cancer cells into a collagen gel during 48 hours. The center channel of a sticky-Slide Chemotaxis was increased in height by milling in order to position the MCF-7 spheroid. The left side contains 10% fetal calf serum, the right side is serum-free. Phase contrast microscopy, objective lens 4x.

    Technical Drawing

    Technical drawings and details are available in the Instructions (PDF).

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